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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10101^* Cite

Description

Cultured retinal pigment epithelial cell were stably transfected with mCherry-clathrin light chain and EGFP-epsin. Live-cell time lapse images were collected on a Nikon TiE TIRF with a 100x1.49 NA objective (~67nm/pixel) using 120 ms exposures. This single dual color image part of an image series that contains the time lapse for the mCherry-clathrin light chain and the EGFP-epsin movies as separate files.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: epithelial cell
Cell Line: ARPE-19
Cellular Component: epsin; clathrin light chain

Attribution

Names: Allen Liu; Sandra L. Schmid

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL10101
Archival Resource Key (ARK): ark:/b7295/w9cil10101

Grouping This image is part of a group.

Imaging

Image Type: recorded image
Image Mode: TIRF
Parameters Imaged: fluorescence emission
Source of Contrast: distribution of a specific protein
Visualization Methods: mCherryFP; EGFP
Processing History: unprocessed raw data
Data Qualifiers: raw, unprocessed data; suitable for spatial measurements

Sample Preparation

Methods: living tissue
Relation To Intact Cell: dispersed cells in vitro

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	67nm
Y	1024px	67nm

Channel	Wavelength
1	350, 488nm