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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10411*  Cite 
Description

DVD quality video of the Contractile Vacuole Complex of Paramecium multimicronucleatum. In vivo CVC filling/expulsion cycles. A CV in a compressed cell is observed in side view undergoing cycles of filling (diastole) and expulsion (systole) of its fluid content as the CV membrane fuses then separates from the plasma membrane and with one radial arm. The radial arm fuses with the CV after systole then just before systole it again separates from the CV. As the CV empties fluid will flow into the end of the radial arm next to the CV that is called the ampullus. This fluid comes from the collecting canal of the same radial arm. There are actually 5 to 10 radial arms connected to one CV but the others are out of the focus plane or out of the field of focus. Movies were taken by Dr. Tomomi Tani with differential interference contrast optics attached to a Leica DMIRB inverted microscope. A Leica Fluotar x63 objective lens (NA = 0.7) was used.

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
contractile vacuole
Biological Context
Biological Process
cellular water homeostasis
Attribution
Names
Tomomi Tani & Richard Allen
Pubmed
10607534
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10411
Archival Resource Key (ARK)
ark:/b7295/w9cil10411
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
compressed living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 720px ——
Y 480px ——