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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10452*  Cite 
Description

60 nm thick section of whole cell of budding yeast prepared by high-pressure freezing, freeze-substitution in acetone containing 1% glutaraldehyde and 0.1% uranyl acetate, and embedding in Lowicryl HM20 resin. Sections were stained with uranyl acetate and lead citrate, imaged with a Tecnai 12 TEM operated at 120 kV, and recoded with a Gatan Ultrascan 1000 CCD. Contrast was digitally adjusted.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae YJM789
Attribution
Names
Christopher Buser
Pubmed
20946827
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10452
Archival Resource Key (ARK)
ark:/b7295/w9cil10452
Grouping This image is part of a group.
Imaging
Image Type
charge coupled device (CCD)
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
lead salt
Processing History
contrast-enhancing methods
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
Methods
cryofixed tissue
freeze substitution
tissue in plastic embedment
glutaraldehyde fixed tissue
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1938px 2nm
Y 1661px 2nm