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Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10512*  Cite 
Description

A monolayer of human foreskin fibroblast cells was infected with Toxoplasma gondii expressing EGFP-tagged TgCentrin2 and mCherry-tagged TgIMC1 (innermembrane complex scaffold protein). Images acquired using API DeltaVision on a Olympus IX70 inverted microscope. Objective: Olympus 100X oil immersion, 1.35 NA. Filters: EGFP, ex 450-490 em 500-540, mCherry, ex 555-592 em 600-665. Dichromatic mirror: Chroma 89021. Captured with CoolSnapHQ/ICX285. Data stack corresponds to CIL# 10511. Unpublished image that is similar to images published in PMID: 16518471. See publication for other experimental details.

Biological Sources
NCBI Organism Classification
Toxoplasma gondii RH
Cell Line
Toxoplasma gondii RH stable cell line
Cellular Component
inner membrane complex
centriole
polar ring of apical complex
Biological Context
Biological Process
regulation of cell shape
cell division
Human Disease
toxoplasmosis
Attribution
Names
John Murray (University of Pennsylvania)
Ke Hu (Indiana University, Bloomington)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL10512
Archival Resource Key (ARK)
ark:/b7295/w9cil10512
Grouping This image is part of a group.
Imaging
Image Type
recorded image
charge coupled device (CCD)
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
mCherryFP
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 512px 0.0663µm
Y 512px 0.0663µm
Z 17px 0.3µm