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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:10602*  Cite 

Macrophage cell line cell infected with Leishmania mexicana. Five amastigotes can be seen within a single large parasitophorous vacuole in the macrophage. Raw 264.7 cells (a leukaemic mouse macrophage line) were infected with amastigote form Leishmania mexicana (WHO strain MNYC/BZ/62/M379) at an MOI of 10 and maintained in RPMI for 4 days. Cells were fixed with 2% paraformaldehyde, and DNA was labelled with propidium iodide following incubation with 50 ug/ml RNaseA for 30 min at room temperature. Z stack of 93 images with a z spacing of 0.194 um captured with a 100x/1.4 NA objective lens on a DM5500 B epifluorescence microscope (Leica) with an Orca cooled CCD camera (Hamamatsu). Note there is a phase channel image that is not currently displayed, but can be turned on in the full viewer mode.

Biological Sources
NCBI Organism Classification
Leishmania mexicana
Mus musculus
Cell Type
Cell Line
WHO strain MNYC/BZ/62/M379
Cellular Component
parasite-containing vacuole
Biological Context
Biological Process
amastigote form Leishmania mexicana
parasite entry into host cell forming a parasitophorous vacuole
Richard Wheeler
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
optical path length gradient
Source of Contrast
differences in adsorption or binding of stain
boundaries between regions with different refractive index
Visualization Methods
propidium iodide
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
permeabilized tissue
Spatial Axis Image Size Pixel Size
X 640px 0.0641µm
Y 480px 0.0641µm
Z 93px 0.194nm
Channel Wavelength
1 dic, fluorescencenm