High voltage TEM image of meiotic newt oocyte nuclei showing transcription on looped lampbrush chromosomes. This micrograph, was recorded at a tilt angle of 40 degrees. The image is grouped with one of the same sample area recorded at a 50 degree tilt. A stereo examination of the pair provides an oblique 3D view.
Oocytes were allowed to disperse in 30 mM KCl/NaCl, centrifuged through 2% formaldehyde onto an EM grid, stained with uranyl acetate, and critical point dried. Grids were examined with the Madison 1MeV TEM at 5 KX.
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