The movie illustrates two approaches to understanding the 3D structure of complex objects, using the pluteus stage embryo of the sea urchin Arbacia punctulata. The first sequence is a through-focus DIC series presented as a 'walk through' in the z dimension. The second uses projections at different angles through a z-stack, with the results presented as a 'rocking' series in which the 3D structure becomes more readily apparent.
See: K Okazaki and S Inoue (1976). Crystal properties of the larval sea urchin spicule. Dev Growth Diff 18:413-434; S Inoue and T Inoue (1986) Computer-aided stereomicroscopic video reconstruction and serial display from high-resolution light microscope optical sections. Ann NY Acad Sci 483:392-404.
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