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CIL:12029*  Cite 

This high resolution micrograph of Paramecium multimicronucleatum is adapted with permission from Fig. 1 in J. Cell Biol. 49:1-20, 1971. Paramecium is covered with cilia which arise from regular surface depressions roughly rectangular in shape. The plasma membrane covers the exterior of the cell and this is closely attached to a mosaic of alveolar sacs internally which in this micrograph are expanded. Borders of adjacent alveolar sacs form septa along each row of cilia and sometimes laterally as well. Septa do not follow the crests of the ridges. The ridges of the rectangular depressions at their tips are filled internally with a granulo-fibrillar material. Trichocyst tips penetrate into the transverse margins along each row of cilia. In this micrograph the trichocysts have been expelled and in a few cases only their membranous casings remain. Collidine buffered fixative. TEM taken on 12/20/68 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
plasma membrane
cell cortex
Richard Allen (University of Hawaii)
J. Cell Biol. 49:1-20, 1971
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 6106px 0.94nm
Y 4768px 0.94nm