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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12071*  Cite 
Description

This high resolution EM features the striated bands that link the membranes under the surface depressions of the cortex. The bands insert into the epiplasm, and in this case, they separate to pass around the parasomal sacs which lie to the right of a row of basal body/cilium complexes. The bands have relatively electron transparent zones next to the epiplasm that lie next to more opaque zones which are in turn separated by a central medium electron transparent zone. This differential banding suggests an overlap of filaments in the two more opaque zones, but there is no evidence of a muscle-like actomyosin system. These bands probably provide tension and keep the cortical ridges from flattening. If contractile they could account for the cell’s ability to alter its shape when swimming through constricted regions. For details on the striated bands in Paramecium see Allen, J. Cell Biol. 49:1-20, 1971 and Allen, Aihara and Fok, J. Euk . Microbiol. 45:202-209, 1998. This image is adapted with permission from Fig. 14 in J. Cell Biol. 49:1-20, 1971. TEM taken on 12/18/68 by R. Allen with Philips 300 operating at 60kV. Neg. 16,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cell cortex
cortical cytoskeleton
cilium
microtubule basal body
cortical striated fiber
Attribution
Names
Richard Allen (University of Hawaii)
Published
J. Cell Biol. 49:1-20, 1971
Pubmed
4102004
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12071
Archival Resource Key (ARK)
ark:/b7295/w9cil12071
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 6124px 0.94nm
Y 4768px 0.94nm