Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Metaphase localization of CENP-E(green) in INCENP OFF cells expressing TrAP-INCENPW766G (red). DAPI (DNA) is blue. See Fig 8D panels 1-4 in Xu et al. 2009 J Cell Biol 187, 637-653.
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