Cell of the DT40 chicken B lymphocyte line at mitosis. Z-series of widefield immunofluorescence images captured using a 100x/1.4NA planapo objective lens on a Deltavision system and deconvolved with a constrained iterative algorithm (Applied Precision). Displayed is the central z-slice. Prometaphase localization of INCENP (red) in INCENP-OFF cells expressing TrAP-INCENP W766G. Endogenous Borealin is green and DAPI (DNA) is blue. See Supplementary Fig 2A panels 9-12 in Supplementary data for Xu et al. 2009 J Cell Biol 187, 637-653.
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