A dividing Didinium nasutum. This large ciliate is capable of engulfing Paramecium. It has a thick ectoplasm containing numerous mucocysts embedded in its extensive epiplasm. Its external surface is coated with a layer of bacteria in this dividing cell. The cytoplasm is relatively compact under the conditions of fixation used. Electron opaque vacuoles are scattered in the cytoplasm. (For a more detailed report see Wessenberg and Antipa, Protistologica 4:427-447, 1968). TEM taken on 5/9/69 by M. Sage with Philips 300 operating at 60kV. Neg. 3,120X. Bar = 2µm.
The negative was printed to paper and the image was scanned to Photoshop. This digitized image is available for qualitative analysis. A high resolution view suitable for qunatitative analysis is available in the library (CIL:38862). Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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