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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12350*  Cite 
Description

High resolution image of the somatic basal bodies along the sides of the cytoproct ridge have bundles of microtubules extending into the endoplasm from near their proximal ends. These bundles make contact with the spent vacuole and aid its movement to the cytoproct. The plasma membrane and alveolar sacs were ruptured in this cell during preparation for electron microscopy. TEM taken on 6/13/79 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 10,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).

Biological Sources
NCBI Organism Classification
Paramecium multimicronucleatum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cellular Component
cytoproct
cell cortex
Biological Context
Biological Process
defecation
cortical cytoskeleton organization
Attribution
Names
Richard Allen (University of Hawaii)
Link
http://www5.pbrc.hawaii.edu/allen/
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL12350
Archival Resource Key (ARK)
ark:/b7295/w9cil12350
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4171px 1.5nm
Y 4912px 1.5nm