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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:124*  Cite 
Description

Telomeres isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Sample was also labeled for the telomere protein TRF1 using immunogold. Image recorded with FEI Tecnai 12 TEM at 100KV and recorded on 2048x2048 CCD with 2x binning. Telomeres have a looped conformation, which likely aids in their protection from the rapid degradation that occurs with free DNA ends.

Biological Sources
NCBI Organism Classification
Gallus gallus gallus
Cell Type
erythrocyte
Cellular Component
chromosome, telomeric region
Biological Context
Biological Process
chromatin assembly
Molecular Function
structural constituent of chromatin
Attribution
Names
woodcock, christopher
Pubmed
2172301
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL124
Archival Resource Key (ARK)
ark:/b7295/w9cil124
Imaging
Image Type
transmission electron microscopy (TEM)
Image Mode
illumination by electrons
detection of electrons
charge coupled device (CCD)
Parameters Imaged
electron density
Source of Contrast
elastic scattering
Visualization Methods
uranyl salt
uranium compound
gold
Processing History
unprocessed raw image
Sample Preparation
Methods
glutaraldehyde fixed tissue
Relation To Intact Cell
isolated subcellular component
Dimensions
Spatial Axis Image Size Pixel Size
X 2048px 0.454nm
Y 2048px 0.454nm