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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:12591*  Cite 

Micrograph of a semi-thick (1-2 µm) section of an infected maize leaf, with silver-enhanced immunogold labeling, that shows dark staining of corn stunt spiroplasma (CSS, Spiroplasma kunkelii, Class Mollicutes) aggregates in phloem tissue. The section was counter stained with toluidine blue. CIL:19129, from the same image group, is a labeled version of this. CSS is a wall-less procaryote that causes corn stunt disease and is biologically transmitted by some leafhopper species, e.g. Dalbulus maidis and D. gelbus (Hemiptera, Cicadellidae). Spiroplasma cells are limited by a unit membrane with no cell wall. They can be quasi-spherical, pleiomorphic or maintain a filamentous/spiral form. CSS multiplies in both plant and insect hosts and is transmitted with salivary secretions during feeding of the vector on new host plants. dditional related images from this contributor are available in the Library.

Biological Sources
NCBI Organism Classification
Spiroplasma kunkelii
Zea mays
Cell Type
plant cell
prokaryotic cell
Cellular Component
plant-type cell wall
Biological Context
Biological Process
El-Desouky Ammar
Saskia A. Hogenhout
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
bright-field microscopy
Image Mode
widefield illumination
Parameters Imaged
absorption of illumination
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
toluidine blue
Data Qualifiers
processed data
Spatial Axis Image Size Pixel Size
X 379px ——
Y 650px ——