Telomeres isolated from chicken erythrocyte nuclei following restriction endonuclease treatment, and imaged by transmission EM after uranyl acetate negative staining. Specimen was also labeled with immunogold using an anti-TRF1 primary antibody. Telomere chromatin adopts a characteristic looped conformation which likely aids in protection from the rapid degradation that occurs with free DNA ends.
Image recorded with FEI Tecnai 12 TEM at 100KV and recorded on 2048x2048 CCD with 2x binning. See also: T Nikitina and C L Woodcock 2004 Closed chromatin loops at the ends of chromosomes. J Cell Biol 166:161-165.
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