High resolution image of a cell sectioned perpendicular to the surface. The parasomal sac ends internally as a coated pit, coated with clathrin. Where the coated pit has pinched off a clathrin-coated vesicle is formed. When the clathrin is removed an endosome results. Other clathrin-coated vesicles arise from early endosomes. TEM taken on 5/21/70 by R. Allen with Hitachi HU11A operating at 75kV. Neg. 27,750X. Adapted with permission from Fig. 1A published in J. Cell Sci. 101:449-461, 1992. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).