Transmission EM showing tight junctions apically and adherens junctions laterally between cells of the enveloping layer of a 24 hr (prim-5) Zebrafish embryo. Specimens fixed in Karnofsky's (overnight at 4˚C) followed by postfixing in 4% osmium tetroxide in .2M cacodylate for 1h. The resin was LX-112 and the stain was uranyl acetate and lead citrate. This image is part of a series taken by Bryan Crawford while he was at the University of Washington. They are part of the Zebrafish--The Living Laboratory CD made available by Mark Cooper and described in Methods in Cell Biology Volume 77, 2004, Pages 439-457.
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