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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13089*  Cite 
Description

Dual-channel spinning-disk confocal time-lapse movie showing kinetochores (Alexa488 labeled CENP-A antibody) in metaphase (red) relative to poleward microtubule flux. Fluorescent speckle microscopy (FSM) of microtubules and kinetochore imaging during metaphase was performed as described in Maddox et al. (2002, 2003), and with a 100X/1.4 NA Plan Apochromat objective (Nikon) with 2X2 binning in the cooled CCD camera (model ER; Hamamatsu Corporation). MetaMorph® software (Universal Imaging Corp.) was used to control shutters, wavelength selection, image acquisition, and storage. Images were acquired at 5-s intervals. Sequential images of different fluorophores were acquired at 3-15s intervals, depending on the experiment. Paired images from different color channels were within 0.5 s of each other. Movie is video 2 from J Cell Biol 2003 162:377-382.

Biological Sources
NCBI Organism Classification
Xenopus [NCBITaxon:262014]
Cellular Component
spindle microtubule
kinetochore
Biological Context
Biological Process
mitotic metaphase
poleward microtubule flux
Attribution
Names
Paul Maddox
Aaron Straight
Peg Coughlin
Timothy J. Mitchison
Edward D. Salmon
Published
J Cell Biol 2003 162:377-382
Pubmed
12900391
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL13089
Archival Resource Key (ARK)
ark:/b7295/w9cil13089
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
fluorescent speckle microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
X-Rhodamine
Alexa Fluor 488
labeled primary antibody
Processing History
color combine
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
unfixed tissue
Relation To Intact Cell
cell extract
Dimensions
Spatial Axis Image Size Pixel Size
X 454px 0.019µm
Y 397px 0.019µm
Channel Wavelength
1 Alexa488, XRhodaminenm
Time 5 seconds