High resolution image of the decorated spongiome is composed of 50nm tubules that have a pattern of helically wound subunits attached to their cytosolic surfaces. These tubules can be up to one micrometer or more in length. Each tubule ends blindly at one end but at its opposite end it empties into a duct shared by several other tubules. This common duct empties into the smooth spongiome. TEM taken 3/13/92 by R. Allen with Zeiss 10A operating at 80kV. Neg. 19,800X. Adapted with permission from the J. Cell Sci. 108:3163-3170, 1995. The raw film was scanned with an Epson Perfection V750 Pro. This image is best used for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 75nm thickness. Additional information available at (http://www5.pbrc.hawaii.edu/allen/).
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