Off-axis patterns perturb uniaxial migration, while 1D lines promote conversion to fibrillar migration. Effects of encountering a perpendicular fibrillar pattern during fibrillar migration (top) and conversion of 2D to 1D migration. 2D matrices were constructed by uniform coating with extracellular matrix (ECM). 1D surfaces were made by using a 2 photon confocal microscope to pattern a polyvinyl alchohol (PVA) coated coverslip. Following gluteraldehyde quenching, ECM proteins were absorbed onto the PVA surface. Movie is video 7 from JCB 2009, 184:481-490
Videos were recorded on a Axiovert 135TV (Carl Zeiss, Inc.) with a charge-coupled device camera (ORCA II ER; Hamamatsu Photonics). MetaMorph imaging software was used to acquire images and control all hardware. A custom environmental chamber (Lucite) enclosed both time-lapse and TIRF microscopes and maintained cells at 37°C with 10% CO2. Images were collected every 4 min and shown at 15 frames per second.
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