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CIL:13408*  Cite 

Phosphorylated H2AX occurs preferentially in open chromatin. Wildtype mouse embryo fibroblasts were treated with the radiomimetic drug neocarzinostatin (NCS) for 1hour prior to being fixed and immunolabeled against the phosphorylated form of histone H2AX and co-stained with DAPI. MEFs were grown on glass coverslips and treated with 10 ng/ml NCS for 1 h or with 1 μM TSA for 8 h and were treated with 10 ng/ml NCS for an additional hour before being fixed in 2% freshly prepared PFA in PBS for 5 min at room temperature. Cells were immunolabeled using monoclonal antiphospho-H2AX antibody (clone JBW301; 1:1,000; Upstate Biotechnology) and goat anti–mouse AlexaFluor546 secondary antibody (Invitrogen). The cells were labeled for 30 min in PBS containing 100 nM DAPI, rinsed with PBS, and mounted on glass microscope slides in glycerol-based mounting media containing N-propyl gallate antifade (Sigma-Aldrich). A stack of confocal optical slices was captured through the depth of the nucleus (Z-axis) using a 63x Plan-Apochromat (N.A. 1.4) objective lens, an optical slice thickness of 800nm, a Z-step size of 200nm, and X-Y pixel size of 70nm. Individual optical slices were background subtracted and contrast adjusted only by stretching the histogram in a linear manner consistently for the different fluorescence channels. Image corresponds to Fig 6B, left panel in Kim et al. J Cell Biol. 178: 209-218. 2007.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cellular Component
nuclear chromatin
nuclear heterochromatin
Biological Context
Biological Process
DNA repair
Jung-Ae Kim
Michael Kruhlak
Farokh Dotiwala
Andre Nussenzweig
James E. Haber
J Cell Biol. 178: 209-218. 2007
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
formaldehyde fixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 381px 70nm
Y 328px 70nm
Z 20px 200nm