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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13591*  Cite 

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The myoblasts in this image were treated with 300ng/ml insulin-like growth factor II (IGF-II) and 50nM rapamycin (Rap) during differentiation. 0.2 μg/ml follistatin was added to the medium for the last 2 day of differentiation. Follistatin was able to rescue the myotube maturation. This image is the control of different treatments in Figure 9A from JCB 189: 1157-1169, 2010. See also CIL: 13589, 13590.

Technical Details

The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens. Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Cellular Component
muscle myosin complex
Biological Context
Biological Process
myoblast fusion
Molecular Function
insulin-like growth factor II binding
mTOR inhibition
Yuting Sun
Yejing Ge
Jenny Drnevich
Yong Zhao
Mark Band
Jie Chen
JCB 189: 1157-1169, 2010
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
distribution of epitope
Visualization Methods
Fluorescein (FITC)
4',6-diamidino-2-phenylindole (DAPI)
primary antibody plus labeled secondary antibody
Processing History
brightness and contrast adjusted
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
permeabilized tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1392px 0.645µm
Y 1040px 0.645µm