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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13594*  Cite 

The C2C12 cell line, a mouse myoblast line, was used here to study the regulatory factors in myogenic differentiation. After cultured in differentiation medium for 3 days, these cells differentiated into myotubes (Green) containing multiple nuclei (Blue). The C2C12 cells stably expressing FLAG-tagged rapamycin-resistant, kinase-inactive (RR/KI) mTOR were induced to differentiate in the presence of 50nM rapamycin (Rap) for 3 days. Conditioned medium (CM) was collected daily from parental C2C12 cells that had been induced to differentiate 1 day earlier than the RR/KI mTOR cells and fed to the latter with 50nM Rap added. CM was able to rescue the effect of Rap to allow myotube maturation. This image is the control of different treatments in Figure 9B from JCB 189: 1157-1169, 2010. See also CIL: 13592, 13593, 13601.

Technical Details

The image was taken by leica DMI 4000B microscope equipped with a QImaging RETIGA Exi camera and Leica 10X/0.22 lens. Myosin heavy chain (MHC) expressed in myotubes was labeled by MF-20 mouse antibody followed by FITC-anti-mouse IgG secondary antibody. Nuclei were stained by DAPI. MHC and nuclei were pseudocolored in green and blue, respectively.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
Cell Line
Cellular Component
muscle myosin complex
Biological Context
Biological Process
myoblast fusion
Molecular Function
mTOR inhibition
Yuting Sun
Yejing Ge
Jenny Drnevich
Yong Zhao
Mark Band
Jie Chen
JCB 189: 1157-1169, 2010
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
compartmentalization of stain or label
distribution of epitope
Visualization Methods
Fluorescein (FITC)
4',6-diamidino-2-phenylindole (DAPI)
primary antibody plus labeled secondary antibody
Processing History
brightness and contrast adjusted
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
permeabilized tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1392px 0.645µm
Y 1040px 0.645µm