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CIL:13656*  Cite 

HeLa cells transiently expressing GlcNAcT-1-HA-KDEL were fixed, immunolabeled for GlcNAcT-1-HA-KDEL (red) and endogenous alpha-mannosidase II (ManII, green), and examined by fluorescence microscopy. Endogenous ManII displayed different staining patterns, depending upon the level of expression of GlcNAcT-1-HA-KDEL. In this image, ManII is localized to the Golgi. Other images in Fig 6 show ManII localization to the ER (CIL# 13655), or cells in which ManII protein is lost completely (CIL# 13657). Transfected HeLa cells were fixed in 3% paraformaldehyde, blocked with 0.2% Triton X-100, 1% FBS, 0.5% BSA in PBS with Ca[2+] and Mg[2+], followed by incubation in primary antibodies (anti–human ManII rabbit pAb and anti-HA mouse mAb, HA.11 (Covance)) and secondary antibodies (Alexa 568 and Alexa 488) and DAPI (blue) to stain nuclei. Cells were mounted using Fluoromount (SouthernBiotech). Immunofluorescent images were digitally acquired on an inverted microscope (Axiovert 200M; Carl Zeiss, Inc.) coupled to a 12-bit cooled charge-coupled device camera (AxioCam MRm Rev. 3; Carl Zeiss, Inc.) controlled by Axiovision software (AxioVs40, version; Carl Zeiss, Inc.), using a 100x/1.3 NA oil immersion objective (EC Plan-NeoFluar; Carl Zeiss, Inc.), and saved as tiff files (1388 × 1040, 8 bit). All pictures were treated identically using Adobe Photoshop to remove background and adjust contrast. This image corresponds to Figure 6D, middle panel (merge) in J Cell Biol. 190: 893-910, 2010. Images in Fig 6 include CIL#s 13655, 13656, 13657.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
epithelial cell
cervical carcinoma
Cell Line
Cellular Component
Golgi apparatus
integral to membrane
Golgi membrane
endoplasmic reticulum
endoplasmic reticulum-Golgi intermediate compartment
Hung-Hsiang Huang
Pamela Stanley
J Cell Biol. 190: 893-910. 2010.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 1388px 169.333µm
Y 1040px 169.333µm