Alternate header for print version

Attribution Non-Commercial Share Alike:This image is licensed under a Creative Commons Attribution, Non-Commercial Share Alike License. View License Deed | View Legal Code
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13732*  Cite 

Mitochondria in HeLa cells are identified by mito-YFP (green) following treatment with the mitochondrial depolarizing agent CCCP (carbonyl cyanide m-chlorophenyl hydrazone). When fixed cells are permeabilized with 0.25% Triton X-100 (TX-100), both antibodies to cyctochrome c (red), a mitochondrial matrix marker, and the antibody to Tom20 (white), a mitochondrial outer membrane marker, are able to bind their epitopes. Mito-YFP transfected HeLa cells were treated with DMSO for 3 hrs, fixed with 4% paraformaldehyde and permeabilized with 0.25% Triton X-100/PBS. Primary antibodies used were: anti-Cyt c mAb (BD) and anti-Tom20 mAb (BD); secondary antibodies used were: Alexa Fluor 594 and 647. Imaging was performed on an LSM510 Meta (Carl Zeiss, Inc) with a 63x 1.4 NA oil differential interference contrast Plan Apo objective. Image contrast and brightness were adjusted in the LSM image browser (Zeiss). This image corresponds to Fig 4b, bottom row and is part of a differential permeabilization assay with and without CCCP that is further described in Fig 4b of J Cell Biol, 191: 933-942, 2010. Images in Fig 4 include CIL#s 13733, 13734, 13729, 13730, 13731, 13732, 13717, 13718, 13719, 13720, 13721, 13722, 13723, 13724.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
endothelial cell
Cell Line
cervical carcinoma
Cellular Component
mitochondrial outer membrane translocase complex
mitochondrial intermembrane space
Seok Min Jin
Michael Lazarou
Chunxin Wang
Lesley A. Kane
Derek P. Narendra
Richard J. Youle
J Cell Biol, 191: 933-942, 2010.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
brightness and contrast adjusted
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px 0.186µm
Y 512px 0.186µm