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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:13760*  Cite 

Electron micrograph of PC12 cells transfected with control siRNA. PC12 cells were plated onto aclar film discs (Pella) coated with poly-L-lysine, transfected twice with 50 nM control siRNA and fixed with 2.5% glutaraldehyde in calcium-/magnesium-free PBS. The discs were washed three times with 0.1 M sodium cacodylate buffer, pH 7.2, and postfixed for 30 min on ice with 1% osmium tetroxide in cacodylate buffer containing 1.6% potassium ferricyanide. The discs were then washed three times with cacodylate buffer, three times with water, incubated with 0.5% uranyl acetate for 30 min (in the dark), and washed again with water. The samples were dehydrated in a graded series of ethanols while progressively lowering the temperature from 4°C to −40°C then embedded in epon resin. After peeling off the aclar, 60-nm sections were cut and viewed in an electron microscope (FEI Tecnai 12; Phillips) at 120 kV, images were captured with a digital camera at 6,800 magnification. Image is similar to Fig 6C left panel (CIL# 13758) of J Cell Biol. 2010. 191: 1173-1187.

Biological Sources
NCBI Organism Classification
Rattus norvegicus
Cell Type
adrenal gland cell
Cell Line
Cellular Component
transport vesicle
trans-Golgi network transport vesicle
plasma membrane
Biological Context
Biological Process
regulated secretory pathway
Cédric S. Asensio
Daniel W. Sirkis
Robert H. Edwards
J Cell Biol. 2010. 191: 1173-1187.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
transmission electron microscopy (TEM)
illumination by electrons
Parameters Imaged
electron density
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
osmium tetroxide
uranyl salt
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Spatial Axis Image Size Pixel Size
X 2048px 352.7778µm
Y 2048px 352.7778µm