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CIL:13925*  Cite 

Transgenic C57BL/6 mice expressing GFP-LC3 fusion protein, uninjected as control for injection with Resveratrol and/or spermidine. To avoid postmortem autophagy induction, dead mice were immediately perfused with 4% paraformaldehyde (wt/vol in PBS, pH 7.4). Tissues were then harvested and further fixed with the same solution for ≥4 h followed by treatment with 15% sucrose (wt/vol in PBS) for 4 h and with 30% sucrose (wt/vol in PBS) overnight. Tissue samples were embedded in Tissue-Tek OCT compound (Sakura) and stored at −70C. 5-µm-thick tissue sections were generated with a cryostat (CM3050 S; Leica), air dried for 1 h, washed in PBS for 5 min, dried at RT for 30 min, and mounted with Vectashield antifading medium. Confocal fluorescent images were captured using a confocal fluorescence microscope (TCS SP2; Leica) fitted with an Apochromat 40× 1.15 NA immersion objective. Images were acquired with a camera (DFC 350 FX 1.8.0; Leica) using LAS AF software (Leica) and processed with Photoshop (CS2; Adobe) software. Specifically, picture processing involved cropping of representative areas and linear adjustments of contrast and brightness and was performed using Photoshop (with equal adjustment parameters for all pictures); no explicit γ correction was used. Image: Figure 9C, middle left panel (muscle/Co), in Morselli et al. J Cell Biol 192: 615-629

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
skeletal muscle cell
Cellular Component
autophagic vacuole
Biological Context
Biological Process
Eugenia Morselli
Guillermo Mariño
Martin v. Bennetzen
Tobias Eisenberg
Evgenia Megalou
Sabrina Schroeder
Sandra Cabrera
Paule Bénit
Pierre Rustin
Alfredo Criollo
Oliver Kepp
Lorenzo Galluzzi
Shensi Shen
Shoaib Ahmad Malik
Maria Chiara Maiuri
Yoshiyuki Horio
Carlos López-Otín
Jens S. Andersen
Nektarios Tavernarakis
Frank Madeo
Guido Kroemer
J Cell Biol 192: 615-629
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
charge coupled device (CCD)
Image Mode
confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
brightness and contrast adjusted
Data Qualifiers
processed data
Sample Preparation
formaldehyde fixed tissue
embedded tissue
cryostat-sectioned tissue
Relation To Intact Cell
sectioned tissue
Spatial Axis Image Size Pixel Size
X 2048px ——
Y 2048px ——