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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:17451*  Cite 
Description

A transverse section near the mid region of the oral proboscis of Didinium. This micrograph shows the extrusive organelles of the mucocysts adjacent to the plasma membrane and the toxicysts. The pexicysts reside in the central region of the proboscis anterior to this section. A section toward the tip of the proboscis is found at (CIL:17452). Further details available at Wessenberg, H. and Antipa, G. 1968. Studies on Didinium nasutum. I. Structure and ultrastructure. Protistologica 4:427-447. And details of the operation of this structure is available at Wessenberg, H. and Antipa, G. 1970. Capture and ingestion of Paramecium by Didinium nasutum. J. Protozool. 17:250-270. TEM taken in 1966 by G. Antipa with JEM T6S operating at 50kV. Neg. 6,000X. The raw film was scanned with an Epson Perfection V750 Pro. This image is available for quantitative analysis. Standard glutaraldehyde fixation followed by osmium tetroxide, dehydrated in alcohol and embedded in an epoxy resin. Microtome sections prepared at approximately 50nm thickness.

Biological Sources
NCBI Organism Classification
Didinium nasutum
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Ciliated Protist
Cell Line
Carolina Biological Supply Company, NC, U.S.A.
Cellular Component
oral apparatus
plasma membrane
cytoplasm
extrusomes
Biological Context
Biological Process
oral apparatus organization
plasma membrane organization
Attribution
Names
Gregory Antipa (San Francisco State University)
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL17451
Archival Resource Key (ARK)
ark:/b7295/w9cil17451
Grouping This image is part of a group.
Imaging
Image Type
transmission electron microscopy (TEM)
illumination by electrons
Image Mode
detection of electrons
film
Parameters Imaged
electron density
Source of Contrast
stain with broad specificity
Visualization Methods
stain with broad specificity
osmium tetroxide
uranyl salt
lead salt
Processing History
recorded image
film
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Dimensions
Spatial Axis Image Size Pixel Size
X 4974px 1.7nm
Y 5458px 1.7nm