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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:19336*  Cite 

Naegleria fowleri is a free living amoebae that can be an opportunistic pathogen, and in some cases, has been diagnosed as the causative agent of the usually fatal amebic encephalitis. Other opportunistic pathogens are Balamuthia and Acanthamoeba. Naegleria and Acanthamoeba feed by phagocytosis. Balamuthia feed by inserting the tips of their pseudopodia into cells. Balamuthia and Acanthamoeba, are the linked video group. The cells were grown in axenic media. Then they were photographed with a Canon Power Shot G3 with a setting at ISO 50 with an optical microlens at 4X and the quality set at RAW. The camera was attached to the phototube of the microscope with a 3X lens and photographed in phase contrast with a 100X oil immersion objective. Live amoebae were placed in a chamber prepared by inverting a coverslip with/or without cultured mammalian cells onto a 3X1 inch microscope slide with a thin rim of Vaseline; melted paraffin was brushed around the edge of the coverslip to seal it to the slide. Such cultures could be maintained for several days at room temperature.

Biological Sources
NCBI Organism Classification
Naegleria fowleri
Cell Type
cell by organism
eukaryotic cell
Eukaryotic Protist
Amoeboid Protist
Cellular Component
Thelma Dunnebacke
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
in-focus phase contrast
Image Mode
phase contrast microscopy
Parameters Imaged
boundaries between regions with different refractive index
Source of Contrast
boundaries between regions with different refractive index
Visualization Methods
natural boundaries
Processing History
living tissue
recorded image
charge coupled device (CCD)
Data Qualifiers
raw, unprocessed data
Sample Preparation
living tissue
living tissue
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 320px ——
Y 240px ——