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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:21755*  Cite 

Five different samples of Drosophila melanogaster Kc167 cells were stained with Hoechst 33342, a DNA stain. The last sample (labeled nodsRNA, CIL 21789) is of wild-type cells. Each of the other four samples (labeled 48, 340, Anillin, and mad2, CIL 21749, 21739, 21759, and 21773, respectively) has a different gene knocked down by RNAi. This is the set that were treated with 48 RNAi. There are 10 images of each sample, for a total of 50 images. The images were acquired on a Zeiss Axiovert 200M microscope. A recommended use for these five image sets is to algorithmically compute the number of cells in each image. A tab-delimited text file containing this information can be found at the URL indicated in the attribution box. The recommended citation when using these images is: "We used the Drosophila Kc167 1 image set (Carpenter, et al., Genome Biology, 2006) available from the Broad Bioimage Benchmark Collection ("

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
Cell Line
Cellular Component
Biological Context
Biological Process
dsRNA 48 treatement
Anne Carpenter
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of DNA
Visualization Methods
Hoechst 33342
Processing History
unprocessed raw data
Sample Preparation
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 512px ——
Y 512px ——