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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:22708*  Cite 
Description

GMPCPP abolishes TIP activity of the CLIP-170(H2)–EB1 complex. The movie shows the localization of CLIP-170(H2)–GFP molecules (a carboxyl truncation of the plus end microtubule tracking protein CLIP-170) on GMPCPP microtubules in the presence of unlabeled EB1 (a plus end microtubule tracking protein). In the absence of GTP hydrolysis, the CLIP-170(H2)–EB1 complex does not specifically target growing microtubule plus-ends, but instead binds along the length of the microtubule lattice. (Scale bar, 2 micron.) Microtubule dynamics were visualized at 22 °C using a TIRF microscope fitted on an inverted microscope (Nikon TE-2000U). Images were captured with a backilluminated electron-multiplying CCD camera (Cascade-512B, Photometrics) using time-lapse capture at burst mode at 10 frames per second for the analysis of +TIP dynamics at the single-molecule resolution. This movie corresponds to Video S4 from Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):492-7. Epub 2009 Jan 6.

Biological Sources
Cellular Component
microtubule
microtubule plus end
EB-1
CLIP-170(H2)
Biological Context
Biological Process
microtubule polymerization or depolymerization
Attribution
Names
Ram Dixit
Brian Barnet
Jacob E. Lazaru
Mariko Tokit
Yale E. Goldman
Erika L. F. Holzbaur
Published
Proc Natl Acad Sci U S A. 2009 Jan 13;106(2):492-7. Epub 2009 Jan 6.
Pubmed
18227130
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL22708
Archival Resource Key (ARK)
ark:/b7295/w9cil22708
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
TIRF
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Rhodamine
EGFP
Processing History
unprocessed raw data
Sample Preparation
Methods
in vitro reconstitution
Relation To Intact Cell
cell free
Dimensions
Spatial Axis Image Size Pixel Size
X 330px 0.036µm
Y 253px 0.036µm
Time 0.01 seconds 90