Association of plectin with myosin II. A gelsolin-treated REF-52 cytoskeleton immunogold labeled myosin II (10 nm). After actin depletion, intermediate filaments with plectin sidearms remain associated with individual myosin bipolar filaments. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10d from J Cell Biol. 1996 Nov;135(4):991-1007.
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