Association of plectin with myosin II. Platinum replica of nocodazole and treated REF-52 cell with actin depleted by gelsolin and plectin labeled with immunogold (10 nm). Individual plectin molecules in lamella cross-link myosin filaments and their clusters. Some plectin molecules bind to each other. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 10e from J Cell Biol. 1996 Nov;135(4):991-1007.
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