Plectin sidearms on intermediate filaments labeled with plectin immuno-gold (10 nm) in REF-52 cells with actin removed by gelsolin treatment. Untreated control appears as CIL: 234044. Electron microscopy of cytoskeletons was performed as described (Svitkina et al., 1995). Briefly, cells on coverslips were lysed as for light microscopy, treated, with recombinant gelsolin NHz-terminal domain, fixed with glutaraldehyde, tannic acid and uranyl acetate, critical point dried, and coated with platinum and carbon. Image corresponds to Fig 1c in J Cell Biol. 1996 Nov;135(4):991-1007.
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