Multiple branching of actin filaments in lamellipodia of vertebrate fibroblasts. This image shows an overview of the leading edge, and CIL 24789 shows enlargements of local regions of this platinum replica. CIL 24786 shows similar overview and local enlargements for the lamellipodia of a Xenopus keratocyte. Image corresponds to Figure 1h from J Cell Biol. 1999 May 31;145(5):1009-26.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
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