Electron micrograph of keratocyte or fibroblast lamellipodial actin network after latrunculin a (a chemical that binds monomeric actin) treatment (0.2 μM for 10 min). All examples demonstrate frequent branching of actin filaments. Image corresponds to Figure 2c in J Cell Biol. 1999 May 31;145(5):1009-26.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
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