Localization of Xenopus ADF/cofilin (XAC) to posterior regions of depolymerization-resistant actin brush. Electron (micrograph of lamellipodia of Xenopus keratocytes after unprotected extraction and subsequent staining with XAC antibody. Image corresponds to Figure 9a from J Cell Biol. 1999 May 31;145(5):1009-26.
Procedures for detergent extraction, immunostaining, S1 decoration, light, and EM were described previously (Svitkina et al., 1995, 1996, 1997;Verkhovsky et al., 1995; Svitkina and Borisy, 1998).
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