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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:24819*  Cite 

Intracellular localization of GFP-GOLPH3 in BY4742 vps74∆ sac1∆. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-GOLPH3 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM. Control image for images showing ectopic expression of GFP-GOLPH3 with mutations in the sulfate-binding pocket (CIL#s 24820, 24821). This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A GFP-GOLPH3 wild-type/sac1∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821.

Biological Sources
NCBI Organism Classification
Saccharomyces cerevisiae S288c
Cell Line
BY4742 vps74∆ sac1∆
Cellular Component
Golgi apparatus
extrinsic to membrane
integral to endoplasmic reticulum membrane
integral to Golgi membrane
Christopher S. Wood
Karl R. Schmitz
Nicholas J. Bessman
Thanuja Gangi Setty
Kathryn M. Ferguson
Christopher G. Burd
J Cell Biol. 187: 967-975. 2009
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Image Type
recorded image
Image Mode
fluorescence microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
Processing History
constrained iterative deconvolution
Data Qualifiers
processed data
suitable for spatial measurements
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 302px 0.0663µm
Y 302px 0.0663µm
Z 11px 0.4µm