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Licensing
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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25328*  Cite 
Description

Salivary glands from 3rd instar larvae of Drosophila melanogaster were dissected, fixed with formalin and stained with uranyl acetate, embedded in epoxy resin, and 0.25 micrometer sections prepared (see Ris H, 1981 Stereoscopic Electron Microscopy of Chromosomes, Meth Cell Biol 22:77-96). Grids were observed using the Madison High Voltage Electron Microscope at 1 MeV. Image shows a series of bands and interbands in a polytene chromosome. This image was taken with a tilt angle of 40 degrees. The accompanying grouped image, taken at 55 degrees forms a stereo pair, providing an oblique 3D view of the chromosome.

Biological Sources
NCBI Organism Classification
Drosophila melanogaster
Cell Type
salivary gland
Cellular Component
polytene chromosome
Attribution
Names
Hans Ris
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25328
Archival Resource Key (ARK)
ark:/b7295/w9cil25328
Grouping This image is part of a group.
Imaging
Image Type
film
Image Mode
transmission electron microscopy (TEM)
Parameters Imaged
elastic scattering of electrons
Source of Contrast
differences in adsorption or binding of stain
Visualization Methods
uranyl salt
Processing History
unprocessed raw data
Sample Preparation
Methods
formaldehyde fixed tissue
tissue in epoxy resin embedment
Relation To Intact Cell
microtome-sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 3862px 1nm
X 2665px 1nm