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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25358*  Cite 
Description

Example of build-up of FPALM image data from a live fibroblast cell expressing PA-GFP-HA. The data analysis routine identifies single molecules, localizes (green boxes) or rejects (yellow and red boxes) them, and builds up a map of plotted positions of molecules, weighted by the number of photons detected from each molecule. Red boxes indicate that the observed object within the box was not large or bright enough (too few pixels above a threshold) to decisively be a single molecule. Yellow boxes indicate that the object within the box was too large (too many pixels above a threshold) to be a single molecule. Frames are approximately 0.19 sec apart in real time. The full width of each window is 53.2 um, including a 5-pixel pad on either side of the actual image. Video corresponds to Supplementary Video 2 from Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17370-5. Epub 2007 Oct 24. See manuscript for details on fPALM imaging and single molecule localization techniques.

Biological Sources
NCBI Organism Classification
Mus musculus
Cell Type
fibroblast
Cell Line
HAb2
Cellular Component
plasma membrane
Biological Context
Biological Process
hemagglutin influenza transfection
Attribution
Names
Samuel T. Hess
Travis J. Gould
Manasa V. Gudheti
Sarah A. Maas
Kevin D. Mills
Joshua Zimmerberg
Published
Proc Natl Acad Sci U S A. 2007 Oct 30;104(44):17370-5. Epub 2007 Oct 24
Pubmed
17959773
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25358
Archival Resource Key (ARK)
ark:/b7295/w9cil25358
Imaging
Image Type
recorded image
Image Mode
fluorescence microscopy
point-localization methods
fPALM
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
PA-GFP
Processing History
single molecule localization
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
living tissue
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 434px 0.25µm
Y 344px 0.25µm
Time 0.19 seconds 51