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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25566*  Cite 

Bacillus subtilis growing in culture. On the left, we see all the cells in a colony. On the right, we see only the cells in normal growth phase because these cells express a green fluorescent protein. After the normal exponential growth phase, there is a "diauxic shift" when growth slows and many of the cells sporulate. This image corresponds to supplemental movie 3 from PNAS 105(11), 4393-4398, 2008.

Technical Details

Microscope slide with live cells in media was incubated in a temperature-controlled Cube and Box incubation system (Life Imaging Services) automated microscope (Zeiss 200M) at 30°C. Images were obtained with a CoolSNAP HQ (Princeton Instruments) at a magnification of either 63X or 100X. Metamorph microscope control software (Universal Imaging) was used. Phase-contrast and fluorescent images were recorded every 5 min for the first 122 frames and every 12 min for the remainder of the time lapse. To prevent phototoxicity, the excitation light (480-520 nm for 2 s) was limited to 5% of the output of a 100-W Hg-vapor lamp by neutral density filters. Emission wavelengths were 505-565 nm (filters from Chroma).

Biological Sources
NCBI Organism Classification
Bacillus subtilis
Cell Type
prokaryotic cell
Biological Context
Biological Process
Jan-Willem Veening
Eric J. Stewart
Thomas W. Berngruber
Francois Taddei
Oscar P. Kuipers
Leendert W. Hamoen
PNAS 105(11), 4393-4398, 2008.
Figure as published
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Sample Preparation
unfixed tissue
Relation To Intact Cell
dispersed cells in vitro
Spatial Axis Image Size Pixel Size
X 984px ——
Y 488px ——
Time 720 seconds 17