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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25572*  Cite 
Description

Time-lapse movies with microscopy by phase contrast (left) and fluorescence (right) taken at 8 minute intervals of typical microcolonies of the strains IIA-gfp/DrapA (top: PspoIIA-gfp, DrapA) and IIA-gfp/Xrap (bottom: PspoIIA-gfp, amyE::Pxyl-rap60) of Bacillus subtilis. The two populations were grown simultaneously in neighboring wells to assure equivalent environmental conditions for comparing growth. The cells begin to express GFP (Green Fluorescent Protein), as seen on the right, at approximately 6 hours. This image corresponds to supplemental movie 9 from PNAS 105(11), 4393-4398, 2008.

Technical Details

The microscope chamber slide was incubated in a temperature-controlled Cube and Box incubation system (Life Imaging Services) automated microscope (Zeiss 200M) at 30°C Images were obtained with a CoolSNAP HQ (Princeton Instruments) at a magnification of either 63X or 100X at eight minute intervals. To prevent phototoxicity, the excitation light (480-500 nm for 0.1 s for GFP) was limited to 32% of the output of a 100-W Hg-vapor lamp by neutral density filters. Emission wavelengths for GFP were 509-547 nm.

Biological Sources
NCBI Organism Classification
Bacillus subtilis
Cell Type
prokaryotic cell
Biological Context
Biological Process
reproduction
cell division
Attribution
Names
Jan-Willem Veening
Eric J. Stewart
Thomas W. Berngruber
François Taddei
Oscar P. Kuipers
Leendert W. Hamoen
Published
PNAS 105(11), 4393-4398, 2008.
Pubmed
18326026
Link
Published article
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25572
Archival Resource Key (ARK)
ark:/b7295/w9cil25572
Sample Preparation
Methods
unfixed tissue
Relation To Intact Cell
living tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1104px ——
Y 1292px ——
Time 480 seconds 25200