Alternate header for print version


Licensing
Public Domain: This image is in the public domain and thus free of any copyright restrictions. However, as is the norm in scientific publishing and as a matter of courtesy, any user should credit the content provider for any public or private use of this image whenever possible. Learn more
tweet  
*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:25693*  Cite 
Description

Time series of cell growth and division in cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) using phase contrast optics. A movie created with this series is grouped with it in the Cell Image Library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 20x HC PL FLUOTAR NA 0.5 objective lens. images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using Simple PCI 6.6.0.0 software.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
retinal pigment epithelium
Cell Line
hTERT-RPE1
Biological Context
Biological Process
mitosis
cell proliferation
Attribution
Names
Toshiyuki Hatano
Greenfield Sluder
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL25693
Archival Resource Key (ARK)
ark:/b7295/w9cil25693
Grouping This image is part of a group.
Sample Preparation
Methods
living tissue
Relation To Intact Cell
whole mounted tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 1344px 0.322µm
Y 1024px 0.322µm
Time 180 seconds 486