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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:26574*  Cite 
Description

Immunofluorescence images used to investigate stabilization of microtubules (MTs) by Von Hippel-Lindau tumor suppressor protein (pVHL). VHL-deficient RCC-4 cells expressing wild-type VHL30 protein have a higher alpha-tubulin (MTs; green) signal relative to GSK-3beta (red; an internal fluorescence control), compared with RCC-4 control cells, suggesting that VHL stabilizes MTs. RCC-4 cells were infected with a retroviral vector to express pVHL30, fixed in 3% paraformaledehyde and permeabilized in 0.2% Triton X-100. Primary antibodies used were: monoclonal anti-GSK3beta and polyclonal anti-alpha tubulin. Secondary antibodies used were: anti-rabbit FITC and anti-mouse Texas-Red. Stacks of 5 z planes were acquired with a Leica SP2 system using the 63x 1.4NA, Oil, HCX Plan-Apo Objective and the distance between z planes is 1 micron. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--Fig 1C (right panels) and the images of the control are in Fig 1C (left panels) and CIL# 31263.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
renal cell carcinoma
epithelial cell
Cell Line
RCC4
Cellular Component
microtubule
cytoskeleton
Axin-APC-beta-catenin-GSK3B complex
protein complex
Attribution
Names
Claudio R. Thoma
Alexandre Matov
Katrin L. Gutbrodt
Christian R. Hoerner
Zlatko Smole
Wilhelm Krek
Gaudenz Danuser
Published
J Cell Biol. 2010. 190: 991-1003.
Pubmed
20855504
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL26574
Archival Resource Key (ARK)
ark:/b7295/w9cil26574
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
single-spot confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Fluorescein (FITC)
TexasRed
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
formaldehyde fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 1024px 8466.667µm
Y 1024px 8466.67µm
Z 5px 1µm