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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27155*  Cite 
Description

Z-stack from rat brain cortex showing a subset of fluorescent-protein expressing neurons and microglia. Most prominent in this stack is an aggregation of microglial cells surrounded by axons and dendrites coursing though the tissue section. This stack is accompanied by a colorized maximum projection image in this image group. Neurons are expressing soluble YFP using the neuron-specific TH1 promoter. Microglia are expressing soluble GFP (transgene inserted in the Cx3cr1 locus). The z-stack was acquired with an iMIC Andromeda laser spinning disc confocal microscope (http://www.till-photonics.com/Products/andromeda.php), using an Olympus UPlanSApo 60X/NA1.35 objective, and: Andor Clara (Andor) CCD Camera. Both fluorophores were excited with a 488nm laser, and so are not distinguished from each other, with these filters: Laser CleanUp, FF01-446/523/600/677 (Semrock); Dichroic, zt405/490/561/640rpc (Chroma); Emission, FF01-390/482/563/640 (Semrock). .

Biological Sources
NCBI Organism Classification
Rattus
Cell Type
multipolar neuron
microglial cell
Cellular Component
cytoplasm
axon
dendrite
Biological Context
Biological Process
gene expression
Attribution
Names
Sebastian Rhode, TILL Photonics
Sabine Scheibe
Link
http://www.till-photonics.com/Products/androm...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL27155
Archival Resource Key (ARK)
ark:/b7295/w9cil27155
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
spinning disk confocal microscopy
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
EGFP
EYFP
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
Sample Preparation
Methods
formaldehyde fixed tissue
Relation To Intact Cell
sectioned tissue
Dimensions
Spatial Axis Image Size Pixel Size
X 576px 0.107µm
Y 504px 0.107µm
Z 200px 0.1µm