Rough endoplasmic reticulum and Golgi in epidermal cells of Fundulus heteroclitus scales. Epidermal cells undergoing advanced stages of scale development have prominent rough endoplasmic reticulum and Golgi indicating increased protein synthesizing activities. A number of vesicle-like structures are also clustered in the cytoplasm.
Fundulus heteroclitus scales were chemically fixed with 2.5% glutaraldehyde, 2% formaldehyde in 0.1M cacodylate buffer (pH 7.3), then post-fixed in 4% osmium tetroxide and stained en bloc in 1% uranyl acetate. The scales were then dehydrated in a graded series of ethanol and infiltrated with Spurr’s resin. Thin sections of 70 nm were trimmed using a diamond knife and post-stained in uranyl acetate and lead citrate. This micrograph was imaged using a Phillips CM 100 transmission electron microscope at an accelerating voltage of 80 kV.
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