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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:27832^* Cite

Description

Time lapse series showing motility and morphology of cultured hTERT-RPE1 cells (telomerase immortalized human retinal pigment epithelium) arrested in G1 using 0.08 uM nocodazole (Uetake and Sluder 2010, Curr Biol 20:1666-1671). A movie prepared from this time series is grouped in the Cell Image library.

Technical Details

Cells were cultured on glass coverslips in 10% FBS, DMEM/F12 medium plus glutamine, HEPES, penicillin and streptomycin and examined with a Leica DMIRE2 microscope with Leica 10x NA 0.3 phase contrast objective lens. Images were captured at 3 minute intervals with a Hamamatsu ORCA-ER2 CCD camera using HC Image 2.0.1.11 software.

Biological Sources

NCBI Organism Classification: Homo sapiens
Cell Type: retinal pigment epithelium
Cell Line: hTERT-RPE1

Biological Context

Biological Process: cell motility; cell morphology

Attribution

Names: Yumi Uetake; Greenfield Sluder

Citation

Digital Object Identifier (DOI): doi:10.7295/W9CIL27832
Archival Resource Key (ARK): ark:/b7295/w9cil27832

Grouping This image is part of a group.

Imaging

Image Type: charge coupled device (CCD)
Image Mode: phase contrast microscopy
Parameters Imaged: elastic scattering of photons
Source of Contrast: differences in amount of elastic light scattering
Visualization Methods: visualization of contiguous regions
Processing History: unprocessed raw data

Sample Preparation

Methods: living tissue
Relation To Intact Cell: whole mounted tissue

Dimensions

Spatial Axis	Image Size	Pixel Size
X	1344px	0.645µm
Y	1024px	0.645µm

Time	180 seconds	1868