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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:28778*  Cite 

Spinning disk confocal time-lapse imaging of the first mitosis in a C. elegans embryo expressing GFP-KNL-2 in strain OD31. As seen for GFP-CeCENP-A (CIL 28780), discrete foci of the GFP signal within the nucleus coalesce to form holocentric kinetochore plates during prophase. GFP image on left and DIC image on right. After nuclear envelope breakdown, chromosomes appear as paired lines and congress to the metaphase plate. At metaphase, sister kinetochores form two lines facing opposite spindle poles. During anaphase, foci of GFP-KNL-2 were seen to form in the spindle area. The video is ∼16 min long, and the frame is ∼30 µm top to bottom. DIC is shown on the right, and GFP is shown on the left. Images were acquired at 10-s intervals.

Technical Details

Images were acquired via a spinning disk confocal microscope (CSU10; McBain Instruments) mounted on an inverted microscope (TE2000e; Nikon). Strain TH32 coexpressing GFP-histone H2b and GFP–γ-tubulin was imaged using a 60× 1.4 NA plan Apo objective with 1.5× auxiliary magnification and a cooled CCD camera (Orca ER; Hamamatsu) binning 2 × 2. Strain OD31 expressing GFP–KNL-2 was imaged in the same manner without the 1.5× auxiliary magnification.

Biological Sources
NCBI Organism Classification
Caenorhabditis elegans
Cell Type
Cellular Component
Paul S. Maddox
Francie Hyndman
Joost Monen
Karen Oegema
Arshad Desai
J Cell Biol. 2007 Mar 12;176(6):757-63. Epub 2007 Mar 5.
Digital Object Identifier (DOI)
Archival Resource Key (ARK)
Grouping This image is part of a group.
Sample Preparation
living tissue
Relation To Intact Cell
whole mounted tissue
Spatial Axis Image Size Pixel Size
X 504px ——
Y 159px ——
Time 10 seconds 99