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*CIL – Cell Image Library accession number. Please use this to reference an image.

CIL:31262*  Cite 
Description

RCC-4 cell stained with anti-alpha-tubulin (green) and anti-GTP-tubulin (red) antibodies. GTP-tubulin localizes to the microtubule (MT) tip and remnants of GTP-tubulin are found along the entire MT lattice. RCC-4 cells lack Von Hippel-Lindau (VHL) and when pVHL30 is expressed in these cells, a higher frequency of GTP caps and GTP remnants is observed. RCC-4 cells were permeabilized in PEM buffer with glycerol and 0.1% Triton-X100, then incubated with primary antibody hMB11 followed by incubation with secondary antibody. Cells were then fixed in cold methanol for 5 min and stained with the polyclonal anti-tubulin antibody followed by secondary antibody. Stacks of images were acquired with an IX70 Delta Vision Spectris microscope using a 60X NA1.4 DIC oil Plan-Apochromat objective. This stack corresponds to J Cell Biol. 2010. 190: 991-1003--control Fig 5F (left 2 panels) and the experimental with ectopic pVHL30 (right 2 panels in Fig 5F) is CIL# 26572.

Biological Sources
NCBI Organism Classification
Homo sapiens
Cell Type
renal cell carcinoma
epithelial cell
Cell Line
RCC4
Cellular Component
microtubule
cytoskeleton
protein complex
Biological Context
Biological Process
protein polymerization
Molecular Function
GTPase activity
structural molecule activity
protein binding
Attribution
Names
Claudio R. Thoma
Alexandre Matov
Katrin L. Gutbrodt
Christian R. Hoerner
Zlatko Smole
Wilhelm Krek
Gaudenz Danuser
Published
J Cell Biol. 2010. 190: 991-1003.
Pubmed
20855504
Link
http://jcb-dataviewer.rupress.org/jcb/browse/...
Citation
Digital Object Identifier (DOI)
doi:10.7295/W9CIL31262
Archival Resource Key (ARK)
ark:/b7295/w9cil31262
Grouping This image is part of a group.
Imaging
Image Type
recorded image
Image Mode
widefield illumination
Parameters Imaged
fluorescence emission
Source of Contrast
distribution of a specific protein
Visualization Methods
primary antibody plus labeled secondary antibody
Processing History
unprocessed raw data
Data Qualifiers
raw, unprocessed data
suitable for spatial measurements
Sample Preparation
Methods
methanol fixed tissue
detergent permeabilized
Relation To Intact Cell
dispersed cells in vitro
Dimensions
Spatial Axis Image Size Pixel Size
X 960px 0.1103µm
Y 960px 0.1103µm
Z 8px 0.2µm